We study how cells control the translation and stability of mRNAs in
the cytosol, and how this regulation fulfills important biological
functions. We study the fundamental principles and diverse functions
of post-transcriptional regulation in organisms ranging from budding
yeast to mammalian neurons.
High-throughput and unbiased technologies are central to our
work. Techniques such as ribosome profiling allow us to measure
translation globally. We have recently developed APEX-seq, a proximity
labeling strategy for learing the composition and organization of
ribonucleoprotein complexes. The lab is also working on
high-throughput functional approaches to characterize regulatory
proteins and genetic networks.
May 2023 — Welcome to Anna Freitas, an MCB Ph.D. student who joined the lab this spring!
May 2023 — Kendra's
global survey of post-transcriptional regulators across the budding yeast proteome is now published at Nature Structural & Molecular Biology
May 2023 — Paige's work to explore the consequences of rapid, inducible depletion of cap-binding protein in budding yeast is now
posted as a pre-print. She found that eIF4E depletion is surprisingly well tolerated, but leads to dysregulated amino acid metabolism and futile cycles of biosynthesis and degradation.
May 2023 — Wren has updated his pre-print,
Molecular recording of calcium concentration via calcium-dependent protein proximity labeling with exciting new results. Great collaborations with
Helen Bateup to show calcium-dependent labeling in the mouse brain and with
Ruixuan Gao to combine labeling with expansion microscopy.
February 2023 — Lucas' work on a simplified and improved ribosome profiling protocol is now
available as a pre-print! This is a great collaboration with to apply her
ordered two-template relay strategy and create OTTR ribosome profiling.