We study how cells control the translation and stability of mRNAs in the cytosol, and how this regulation fulfills important biological functions. We study the fundamental principles and diverse functions of post-transcriptional regulation in organisms ranging from budding yeast to mammalian neurons.
High-throughput and unbiased technologies are central to our work. Techniques such as ribosome profiling allow us to measure translation globally. We have recently developed APEX-seq, a proximity labeling strategy for learing the composition and organization of ribonucleoprotein complexes. The lab is also working on high-throughput functional approaches to characterize regulatory proteins and genetic networks.
CiBER-seq yields comprehensive and quantitative profiles of transcriptional, translational, or post-translational regulatory responses across genome-scale CRISPR perturbation libraries. We can imagine a lot of uses for CiBER-seq and we look forward to seeing how it's used!